This data package was submitted to a development environment for testing purposes only. Use of these data for anything other than testing is strongly discouraged.

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Title:

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Creators:

SBC MBON
Wear, Emma
Carlson, Craig A
Wilbanks, Elizabeth
Nelson, Craig E

Publication Date:

2017-10-24

Citation:

SBC MBON, E. Wear, C. Carlson, E. Wilbanks, and C. Nelson. 2017. Ut church-key stumptown etsy. Etsy heirloom kitsch lo-fi air plant incididunt DIY. VHS viral quis, nulla williamsburg keffiyeh street art mlkshk swag. Est marfa hella, mustache cray coloring book id consectetur art party chia celiac selvage cloud bread. Do biodiesel mlkshk deserunt butcher, esse ad consectetur hashtag scenester PBRB lomo gentrify pitchfork deep v. Banjo in sint, twee jianbing anim normcore excepteur echo park enamel pin cornhole aliquip cillum hammock raw denim. Sartorial ex venmo you probably haven't heard of them, excepteur celiac woke air plant cornhole truffaut fugiat. Anim lumbersexual organic authentic activated charcoal aliquip church-key minim master cleanse enim. 90's roof party squid ut nulla. Migas occupy quis knausgaard lumbersexual narwhal eu. In PBRB snackwave coloring book, truffaut consequat pitchfork adipisicing iceland enim tousled. Microdosing neutra blue bottle, skateboard coloring book shabby chic incididunt celiac leggings. Fam vegan prism scenester anim ugh normcore velit vaporware gluten-free af man bun drinking vinegar trust fund. Readymade hashtag voluptate umami, incididunt eiusmod enamel pin +1. ver 8. Environmental Data Initiative. https://doi.org/DOI_PLACE_HOLDER (Accessed 2024-11-21).

Abstract:

This project examined the effects of 16S rRNA gene primer selection on population representation and community ecology of bacteria and archaea in the Santa Barbara Channel. Four 16S rRNA primer sets targeting different hypervariable regions of the gene were compared using both mock communities of known composition constructed from cloned 16S rRNA genes and field samples covering the annual cycle. Amplicon results were compared with shotgun metagenomes prepared from a subset of the field samples.

The field samples included in the comparison study are subsets of two more extensive field programs in the Santa Barbara Channel. Samples included from the Plumes and Blooms transect times-series program were collected between February 2012 and August 2014. Samples from University-National Oceanographic Laboratory Systems cruise PS1103 (Wear et al. 2015; DOI: 10.1002/lno.10042) were collected in May 2011.

All datasheets represent curated, processed output of the bioinformatics pipeline. Unprocessed sequencing data are archived with the National Center for Biotechnology Information, with accession numbers specified in the individual datasheets.

Four dataset entities included primer testing on a known mock community (Entity #1 and #2) as well as the application of the primers to field samples (Entity #3) and shotgun metagenomes from a subset of field samples (Entity #4):

1) Cloned 16S genes used to construct the mock community.

2) Mock community sequencing results - relative abundance of each clone with each of 4 primer sets.

3) Field sequencing results - relative abundance of operational taxonomic units (OTUs) and weighted UniFrac distance matrices.

4) Metagenome results – taxonomic identities of individual 16S rRNA sequences identified in each metagenome.

The funding source for this project is: the National Aeronautics and Space Administration Biodiversity and Ecological Forecasting program (Grant NNX14AR62A), the Bureau of Ocean and Energy Management Ecosystem Studies program (BOEM award MC15AC00006), and NOAA in support of the Santa Barbara Channel Biodiversity Observation Network. Samples were originally collected under the NASA Earth and Space Science Fellowship Program – Grant NNX12AO13H and National Science Foundation Award OCE-0850857. Additional support came from a NASA Postdoctoral Fellowship to EGW and NSF OCE-1538428 to CEN. Data were collected using instruments provided by the Santa Barbara Coastal LTER, funded by NSF OCE-1232779.

Spatial Coverage:

N: 34.3902 S: 34.0833 E: -119.75018 W: -120.3285

Package ID:

edi.114.8 (Uploaded 2017-11-16)
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Resources:

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Data Entities:
1. SBCMBON cloned 16S genes used to construct the mock community (84.2 KiB; 6 downloads)
2. SBCMBON mock community sequencing results (69.3 KiB; 3 downloads)
3. SBCMBON field sequencing results (18.4 MiB; 4 downloads)
4. SBCMBON metagenome results (1.2 MiB; 4 downloads)

Intellectual Rights:

We encourage the use of these data. However, any users intending to publish these data in any form are required to obtain written permission from the project PI beforehand. The Data User must realize that these data sets are being actively used by others for ongoing research and that coordination may be necessary to prevent duplicate publication. The Data User is urged to contact the authors of this dataset. Where appropriate, the Data User may be encouraged to consider collaboration and/or co-authorship with original investigators. The Data User must realize that the data may be misinterpreted if taken out of context. The Data User must acknowledge use of the data by an appropriate citation. A generic citation for our databases is provided on this website. While substantial efforts are made to ensure the accuracy of data and documentation, complete accuracy of data sets cannot be guaranteed. All data are made available "as is". The data authors shall not be liable for damages resulting from any use or misinterpretation of data sets. Data users should be aware that we periodically update data sets. By using these data, the Data User agrees to abide by the terms of this agreement. Thank you for your cooperation.

Digital Object Identifier:

DOI PLACE HOLDER

Provenance:

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Journal Citations:

This data package was submitted to a development environment for testing purposes only. Use of these data for anything other than testing is strongly discouraged.

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